秦满+陈峰等：牙菌斑菌群代谢能力与儿童龋齿有啥关系？
- ①纳入32名无龋（CF）和31名患龋（CA）儿童进行分析；
- ②CA组牙菌斑菌群代谢95种单一碳源的综合能力持续高于CF儿童；
- ③CA组牙菌斑菌群的核心碳源数量是CF组的2倍多，包括CF组的全部14个核心碳源，以及CA组特有的蔗糖、葡萄糖和棉子糖等17个致龋碳源；
- ④利用CA和CF组之间在24小时代谢能力有显著差异的10个单一碳源，可有效聚类区分CA和CF儿童；
- ⑤CA和CF儿童的饮食习惯差异明显，CA组断夜奶的年龄更晚，吃夜奶和甜食甜饮料的频率更高。
主编推荐语
与菌群结构和组成相比，菌群代谢潜力或许能更好的反映疾病状态。Frontiers in Microbiology近期发表了来自北京大学附属口腔医院秦满团队与陈峰团队合作、田靖为第一作者的研究，主要分析了患龋儿童和健康儿童的牙菌斑菌群代谢单一碳源的能力差异，发现前者有更为广泛的代谢能力，包括能利用多种致龋碳源。该研究还表明，从口腔菌群代谢层面来区分患龋和无龋个体时，Biolog分析是一个潜在的有效工具。
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Differences in Sole Carbon Source Utilization of the Dental Plaque Microbiota Between Caries-Free and Caries-Affected Children
无龋与患龋儿童的牙菌斑微生物群利用单一碳源的差异
10.3389/fmicb.2020.00458
2020-03-20, Article
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Increasing lines of evidence indicate that while microbial profile might vary, community-level metabolic potential is often more stably correlated with healthy and diseased states. Here, we investigated the community-level metabolic diversity of dental plaque microbiota from caries-free (CF) and caries-affected (CA) children by measuring their sole carbon source utilization using a Biolog assay. The dietary habits of 32 CF and 31 CA children were recorded by a questionnaire. Supragingival plaque samples were collected and inoculated into Biolog AN Microplates to assess the metabolism of sole carbon sources by plaque bacteria. The results revealed significant differences in dietary habits between CF and CA children. Meanwhile, Biolog assay showed consistently higher, albeit not statistically significant, overall metabolic activity as measured by average well color development (AWCD) value in the plaque microbiota from CA group than CF group. Most importantly, the CA group had more than twice as many core-positive carbon sources (defined as being utilized by >90% of plaque microbiota from subjects within the group) as that of the CF group (31 vs. 14), including CA group-specific, cariogenic core-positive carbon sources such as sucrose, glucose and raffinose. Furthermore, CF and CA groups could be well distinguished by cluster and principle component analyses based on the types of sole carbon sources significantly differentially utilized by the two groups. Our results indicate that plaque communities associated with caries state are more metabolically versatile than those associated with healthy state, which could contribute to differential clinical caries states. Meanwhile, Biolog could be an effective tool in revealing the community-level physiological profiles of microbiota associated with different caries states.
First Authors:
Jing Tian
Correspondence Authors:
Feng Chen,Man Qin
All Authors:
Jing Tian,Weihua Shi,He Xu,Guiyan Wang,Xuesong He,Feng Chen,Man Qin
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