炎症性肠炎儿童口腔菌群失调
- ①纳入156名被诊断为炎症性肠病（IBD）的儿童和102名健康对照，检测舌背微生物组；
- ②IBD与健康对照儿童的舌背菌群结构显著不同，其中梭状芽胞杆菌属丰度降低，而链球菌丰度增加；
- ③潜在致病菌（克雷伯菌和假单胞菌）也与儿童IBD存在关联；
- ④这种菌群失调在疾病程度更严重的患者中更加明显；
- ⑤菌群功能分析提示，IBD患者口腔菌群潜在产酸能力增强，而B族维生素合成能力降低；
- ⑥对53名治疗后的患者连续观察，发现口腔失调问题可以被逆转。
主编推荐语
本研究通过病例对照研究，发现患有炎症性肠炎的儿童口腔菌群结构紊乱明显；但是这种紊乱在IBD治疗后有显著的改善。本研究为IBD治疗的口腔菌群干预靶点提供了科学的新的视角。
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The oral microbiome in treatment naïve paediatric IBD patients exhibits dysbiosis related to disease severity that resolves following therapy
未接受过治疗的儿童IBD患者的口腔微生物组表现出与疾病严重程度相关的生态失调，且治疗后会消退
10.1093/ecco-jcc/jjac155
2022-10-14, Article
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Background: There is a limited literature describing the oral microbiome and its diagnostic potential in paediatric inflammatory bowel disease (IBD).
Methods: We examined the dorsum tongue microbiome by V1-V2 sequencing in a cohort of 156 treatment naïve children diagnosed with IBD compared to 102 healthy control children. Microbiome changes over time following treatment were examined in a subset of patients and associations between IBD diagnosis and dysbiosis were explored.
Results: Analysis of community structure of the microbiome in tongue samples revealed that IBD samples significantly diverged from healthy control samples (PERMANOVA P=0.0009) and exhibited a reduced abundance of Clostridia in addition to several major oral genera (Veillonella, Prevotella, Fusobacterium species) with an increased abundance of streptococci. This dysbiosis was more marked in patients with severe disease. Higher levels of the potential pathobionts Klebsiella and Pseudomonas spp. were also associated with IBD. In terms of predicted functions, the IBD oral microbiome was potentially more acidogenic and exhibited reduced capacity for B vitamin biosynthesis. We used a machine learning approach to develop a predictive model of IBD which exhibited a mean-prediction AUC: 0.762. Finally, we examined a subset of 53 patients following 12 months of therapy and could show resolution of oral dysbiosis demonstrated by a shift towards a healthy community structure and a significant reduction in oral dysbiosis.
Conclusion: Oral dysbiosis found in children with IBD is disease severity related and resolves over time following successful IBD treatment.
First Authors:
Khalid Elmaghrawy
Correspondence Authors:
Séamus Hussey,Gary P Moran
All Authors:
Khalid Elmaghrawy,Paddy Fleming,Kirsten Fitzgerald,Sarah Cooper,Anna Dominik,Séamus Hussey,Gary P Moran
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