口腔菌群或与肺癌密切相关？
- ①选取美国3个人群队列，分别随访13.9、6.4和7.9年，最终纳入1306例肺癌患者和3304名健康人，采集口腔清洗样本测序；
- ②发现较高的口腔菌群α多样性与较低的肺癌风险相关；
- ③较高丰度的乏养菌属、乳酸杆菌属和链球菌属，及消化链球菌、尤氏真杆菌、嗜胨菌属和团聚杆菌属的存在与肺癌风险有关；
- ④菌群α多样性与较低的鳞状细胞癌风险相关，但与腺癌和小细胞肺癌关联较弱；
- ⑤口腔菌群与肺癌风险密切相关，尤其对于鳞状细胞癌患者和曾吸烟者。
主编推荐语
多项研究发现，肺癌患者的口腔菌群与健康人存在显著差异，部分物种也有增加肺癌患病风险的可能。近日，美国国家癌症研究所研究人员在Journal of the National Cancer Institute发表最新研究，选取美国3个人群队列约4600多人（1306例肺癌患者和3304名健康人），发现口腔菌群与肺癌密切相关，且较高的微生物多样性与较低的肺癌风险相关。还鉴定出几个标志性物种，尤其是高丰度的乏养菌属、乳酸杆菌属和链球菌属显著增加了患病风险。总之，口腔菌群与肺癌风险密切相关，尤其对于鳞状细胞癌患者和曾吸烟者。
关键字
延伸阅读本研究的原文信息和链接出处，以及相关解读和评论文章。欢迎读者朋友们推荐！
The oral microbiome and lung cancer risk: An analysis of 3 prospective cohort studies
口腔微生物组与肺癌风险：3 项前瞻性队列研究分析
10.1093/jnci/djac149
2022-08-05, Article
Abstract & Authors:展开
Abstract:收起
Background: Previous studies suggested associations between the oral microbiome and lung cancer, but studies were predominantly cross-sectional and underpowered.
Methods: Using a case-cohort design, 1,306 incident lung cancer cases were identified in the Agricultural Health Study, NIH-AARP Diet and Health Study, and Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial. Referent subcohorts were randomly selected by strata of age, sex, and smoking history. DNA was extracted from oral wash specimens using the DSP DNA Virus Pathogen kit, the 16S rRNA gene V4 region was amplified and sequenced, and bioinformatics were conducted using QIIME 2. Hazard ratios (HR) and 95% confidence intervals (CI) were calculated using weighted Cox proportional hazards models.
Results: Higher alpha diversity was associated with lower lung cancer risk (Shannon index HR 0.90; 95% CI: 0.84–0.96). Specific principal component vectors of the microbial communities were also significantly associated with lung cancer risk. After multiple testing adjustment, greater relative abundance of three genera and presence of one genus were associated with greater lung cancer risk, while presence of three genera were associated with lower risk. For example, every standard deviation increase in Streptococcus abundance was associated with 1.14 times the risk of lung cancer (95% CI: 1.06–1.22). Associations were strongest among squamous cell carcinoma cases and former smokers.
Conclusions: Multiple oral microbial measures were prospectively associated with lung cancer risk in three US cohort studies with associations varying by smoking history and histologic subtype. The oral microbiome may offer new opportunities for lung cancer prevention.
First Authors:
Emily Vogtmann,Xing Hua
Correspondence Authors:
Emily Vogtmann
All Authors:
Emily Vogtmann,Xing Hua,Guoqin Yu,Vaishnavi Purandare,Autumn G Hullings,Dantong Shao,Yunhu Wan,Shilan Li,Casey L Dagnall,Kristine Jones,Belynda D Hicks,Amy Hutchinson,J Gregory Caporaso,William Wheeler,Dale P Sandler,Laura E Beane Freeman,Linda M Liao,Wen-Yi Huang,Neal D Freedman,Neil E Caporaso,Rashmi Sinha,Mitchell H Gail,Jianxin Shi,Christian C Abnet
评论
