肖英平+陶诗煜+杨华:丁酸梭菌的胞外囊泡调节肠道稳态并缓解小鼠结肠炎
创作:aluba 审核:aluba 2022年07月05日
  • 在DSS诱导的结肠炎小鼠中,丁酸梭菌可抑制体重减少、降低疾病活动度评分、减小结肠长度、降低组织学评分、改善肠道屏障功能、减少致病菌(埃希氏菌属/志贺氏菌属)、增加产丁酸盐菌;
  • 移植丁酸梭菌处理后的小鼠的粪便菌群或无菌粪便滤液,也可缓解DSS诱导的小鼠结肠炎,说明丁酸梭菌通过调节肠道菌群及代谢产物发挥作用;
  • 丁酸梭菌产生的胞外囊泡可保护肠道屏障功能,改善肠道菌群稳态,从而缓解DSS诱导的小鼠结肠炎。
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aluba
丁酸梭菌可在结肠炎小鼠中起保护作用,但背后的机制尚未明确。浙江省农业科学院的肖英平、杨华与华中农业大学的陶诗煜作为共同通讯作者在Microbiology spectrum上发表的一项最新研究结果,发现丁酸梭菌可通过其胞外囊泡调节肠道菌群及代谢产物,以改善肠道屏障功能,从而缓解DSS诱导的小鼠结肠炎。
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Clostridium butyricum and Its Derived Extracellular Vesicles Modulate Gut Homeostasis and Ameliorate Acute Experimental Colitis

丁酸梭菌及其产生的胞外囊泡调节肠道稳态并缓解急性实验性结肠炎

10.1128/spectrum.01368-22

2022-06-28, Article

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Microbiological treatments are expected to have a role in the future management of inflammatory bowel disease (IBD). Clostridium butyricum (C. butyricum) is a probiotic microorganism that exhibits beneficial effects on various disease conditions. Although many studies have revealed that C. butyricum provides protective effects in mice with colitis, the way C. butyricum establishes beneficial results in the host remains unclear. In this study, we investigated the mechanisms by which C. butyricum modifies the gut microbiota, produces bacterial metabolites that may be involved, and, specifically, how microbial extracellular vesicles (EVs) positively influence IBD, using a dextran sulfate sodium (DSS)-induced colitis murine model in mice. First, we showed that C. butyricum provides a protective effect against colitis, as evidenced by the prevention of body weight loss, a reduction in the disease activity index (DAI) score, a shortened colon length, decreased histology score, and an improved gut barrier function, accompanied by reduced levels of pathogenic bacteria, including Escherichia/Shigella, and an increased relative abundance of butyrate-producing Clostridium sensu stricto-1 and Butyricicoccus. Second, we also confirmed that the gut microbiota and metabolites produced by C. butyricum played key roles in the attenuation of DSS-induced experimental colitis, as supported by the profound alleviation of colitis effects following fecal transplantation or fecal filtrate insertion supplied from C. butyricum-treated mice. Finally, C. butyricum-derived EVs protected the gut barrier function, improved gut microbiota homeostasis in ulcerative colitis, and contributed to overall colitis alleviation.

First Authors:
Lingyan Ma

Correspondence Authors:
Hua Yang,Shiyu Tao,Yingping Xiao

All Authors:
Lingyan Ma,Qicheng Shen,Wentao Lyu,Lu lv,Wen Wang,Minjie Yu,Hua Yang,Shiyu Tao,Yingping Xiao

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