口腔菌群通过影响免疫细胞与骨细胞互作
- ①研究SPF与无菌（GF）小鼠牙槽骨和非口腔骨骼的骨免疫，评估与全身菌群不同的口腔菌群诱导的骨免疫调节作用；
- ②SPF组与GF组小鼠皮质/小梁骨减少，牙槽骨中促破骨表型增强；
- ③SPF小鼠牙槽骨BM中具有促破骨作用的TLR信号和Th17细胞增加，诱导口腔菌群参与特殊免疫刺激作用；
- ④口腔共生菌诱导小鼠牙槽骨BM中MHCII抗原限制性呈递；
- ⑤洗必泰抑菌剂冲洗可减少SPF小鼠口腔菌群（但不影响肠道菌群），有效缓解自然发生的骨质流失，维持牙槽骨稳态。
主编推荐语
共生微生物对骨骼稳态有重要调节作用，但特异性微生物群落对骨免疫反应机制的影响尚不清楚。近日，南卡罗莱纳医科大学研究人员在JCI insight发表最新研究，发现口腔菌群激活了一组免疫细胞，而这些免疫细胞反过来又会影响破骨细胞。进一步动物实验发现使用杀菌漱口水来消耗健康的口腔菌群，可有效缓解自然发生的骨质流失，维持牙槽骨稳态，表明口腔菌群和牙槽骨丢失间有直接联系。未来仍需要深入挖掘哪些特定的物种真正导致了自然发生的牙槽骨流失。
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Commensal oral microbiota induces osteoimmunomodulatory effects separate from systemic microbiome in mice
口腔共生菌群在小鼠体内诱导不同于系统性菌群的骨免疫调节作用
10.1172/jci.insight.140738
2022-01-25, Article
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Commensal microbes critically regulate skeletal homeostasis, yet the impact of specific microbiota communities on osteoimmune response mechanisms is unknown. To discern osteoimmunomodulatory effects imparted by the commensal oral microbiota that are distinct from the systemic microbiota, osteoimmunology studies were performed in both alveolar bone and nonoral skeletal sites of specific pathogen–free (SPF) versus germ-free (GF) mice and SPF mice subjected to saline versus chlorhexidine oral rinses. SPF versus GF mice had reduced cortical/trabecular bone and an enhanced pro-osteoclastic phenotype in alveolar bone. TLR signaling and Th17 cells that have known pro-osteoclastic actions were increased in alveolar BM, but not long BM, of SPF versus GF mice. MHC II antigen presentation genes and activated DCs and CD4+ T cells were elevated in alveolar BM, but not long BM, of SPF versus GF mice. These findings were substantiated by in vitro allostimulation studies demonstrating increased activated DCs derived from alveolar BM, but not long BM, of SPF versus GF mice. Chlorhexidine antiseptic rinse depleted the oral, but not gut, bacteriome in SPF mice. Findings from saline- versus chlorhexidine-treated SPF mice corroborated outcomes from SPF versus GF mice, which reveals that the commensal oral microbiota imparts osteoimmunomodulatory effects separate from the systemic microbiome.
First Authors:
Jessica D Hathaway-Schrader
Correspondence Authors:
Chad M Novince
All Authors:
Jessica D Hathaway-Schrader,Johannes D Aartun,Nicole A Poulides,Megan B Kuhn,Blakely E McCormick,Michael E Chew,Emily Huang,Richard P Darveau,Caroline Westwater,Chad M Novince
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