秦满+何雪松+陈峰：儿童龋齿的纵向口腔菌群变化
- ①追踪3岁时无龋齿儿童12个月，选取6个月时患龋却不治疗的10名儿童，另选19名健康儿童对照，分析龈上菌群变化；
- ②龋病发生发展过程中，龈上菌群结构改变，细菌间互作的数量和强度均显著增加，而对照组无明显变化；
- ③致龋相关细菌间的正相互作用、致龋相关细菌和健康相关细菌间的负相互作用，均伴随龋病增强，说明细菌间的互作在龋病发生发展中有重要意义；
- ④龋病发生前6个月菌群组成的变化就已发生，建立龋病发生的预测模型准确度达93.1%。
主编推荐语
北大口腔医院秦满和陈峰以及哈佛大学福赛斯研究所何雪松共同主导的最新研究，在Frontiers in Microbiology发表。该研究对144名幼儿龋病发生发展进行1年的纵向观察，揭示了龋病发生前后和发展过程中龈上菌群的变化，并根据龋病发生前的菌群特征建立了预测模型，或可作为龋病预测和早期诊断的依据，对临床及基础研究都具有指导意义，推荐专业人士关注。
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Oral Microbiome Shifts From Caries-Free to Caries-Affected Status in 3-Year-Old Chinese Children: A Longitudinal Study
中国3岁儿童从无龋齿向有龋齿变化的口腔微生物组：一项纵向研究
10.3389/fmicb.2018.02009
2018-08-28, Article
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As one of the most prevalent human infectious diseases, dental caries results from dysbiosis of the oral microbiota driven by multiple factors. However, most of caries studies were cross-sectional and mainly focused on the differences in the oral microbiota between caries-free (CF) and caries-affected (CA) populations, while little is known about the dynamic shift in microbial composition, and particularly the change in species association pattern during disease transition. Here, we reported a longitudinal study of a 12-month follow-up of a cohort of 3-year-old children. Oral examinations and supragingival plaque collections were carried out at the beginning and every subsequent 6 months, for a total of three time points. All the children were CF at enrollment. Children who developed caries at 6-month follow-up but had not received any dental treatment until the end of the study were incorporated into the CA group. Children who remained CF at the end of the study were incorporated into the CF group. Using Illumina Miseq Sequencing of the 16S rRNA gene, we monitored the shift of supragingival microbiome during caries initiation and progression in children who developed caries over the 12-month study period. Intriguingly, principle coordinates analyses revealed two major shifting patterns in microbial structures during caries initiation and progression in CA group, but not in CF group. Dynamic co-occurring OTU network study showed that compared to CF group, there was significant increase in both number and intensity of correlations between microbial taxa, as well as the formation of tight clusters of specific bacteria in CA group. Furthermore, there were enhanced correlations, positive ones between CA-enriched taxa, and negative ones between CF-enriched and CA-enriched species within CA group. Our data suggested coordinated microbial interactions could be essential to caries pathogenesis. Most importantly, our study indicated that significant microbial shifts occur not only during caries development, but even in the sub-clinical state. Using supragingival microbiome profiles, we were able to construct a caries-onset prediction model with a prediction accuracy of 93.1%. Our study indicated that the microbial shifts prior to the onset of caries might potentially be used for the early diagnosis and prediction of caries.
First Authors:
He Xu,Jing Tian
Correspondence Authors:
Man Qin,Xuesong He,Feng Chen
All Authors:
He Xu,Jing Tian,Wenjing Hao,Qian Zhang,Qiong Zhou,Weihua Shi,Man Qin,Xuesong He,Feng Chen
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